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rabbit il4 elisa kit  (R&D Systems)


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    Structured Review

    R&D Systems rabbit il4 elisa kit
    Representative fluorescence microscopy images of mesenchymal stromal cells (MSCs) infected by IL-4 and/or PDGF-BB lentiviral vectors. GFP-MSCs, MSCs infected with control GFP-positive lentivirus vector; <t>IL4-MSCs,</t> MSCs infected with rIL-4 secreting GFP-positive lentivirus vector; PDGF-BB-MSCs, MSCs infected with hPDGF-BB secreting GFP-positive lentivirus vector; IL4-PDGF-BB-MSCs, previously established IL4-MSCs infected with hPDGF-BB secreting RFP-positive lentivirus vector.
    Rabbit Il4 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit il4 elisa kit/product/R&D Systems
    Average 93 stars, based on 2 article reviews
    rabbit il4 elisa kit - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro"

    Article Title: Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

    Journal: Bioengineering

    doi: 10.3390/bioengineering8110165

    Representative fluorescence microscopy images of mesenchymal stromal cells (MSCs) infected by IL-4 and/or PDGF-BB lentiviral vectors. GFP-MSCs, MSCs infected with control GFP-positive lentivirus vector; IL4-MSCs, MSCs infected with rIL-4 secreting GFP-positive lentivirus vector; PDGF-BB-MSCs, MSCs infected with hPDGF-BB secreting GFP-positive lentivirus vector; IL4-PDGF-BB-MSCs, previously established IL4-MSCs infected with hPDGF-BB secreting RFP-positive lentivirus vector.
    Figure Legend Snippet: Representative fluorescence microscopy images of mesenchymal stromal cells (MSCs) infected by IL-4 and/or PDGF-BB lentiviral vectors. GFP-MSCs, MSCs infected with control GFP-positive lentivirus vector; IL4-MSCs, MSCs infected with rIL-4 secreting GFP-positive lentivirus vector; PDGF-BB-MSCs, MSCs infected with hPDGF-BB secreting GFP-positive lentivirus vector; IL4-PDGF-BB-MSCs, previously established IL4-MSCs infected with hPDGF-BB secreting RFP-positive lentivirus vector.

    Techniques Used: Fluorescence, Microscopy, Infection, Control, Plasmid Preparation

    The experimental outline of in vitro experiments. GFP-MSCs, IL4-MSCs, PDGF-BB-MSCs, or IL4-PDGF-BB-MSCs were seeded into T75 flasks and cultured in an MSC growth medium for 1 day. For the non-preconditioning MSC groups, the cells were cultured for 3 days with a fresh MSC growth medium. For the preconditioning MSC groups, cells were cultured in the MSC preconditioning medium for 3 days. The cells from all eight groups were washed three times with Dulbecco’s phosphate-buffered saline. The cells were trypsinized and seeded for each experiment, including cell proliferation, IL4 and PDGF-BB expression level, and osteogenic differentiation performed by alkaline phosphatase (ALP) staining and Alizarin red-staining. Abbreviations: MSCs, mesenchymal stromal cells; pMSCs, preconditioning MSCs; TNFα, tumor necrosis factor-alpha; LPS, lipopolysaccharide.
    Figure Legend Snippet: The experimental outline of in vitro experiments. GFP-MSCs, IL4-MSCs, PDGF-BB-MSCs, or IL4-PDGF-BB-MSCs were seeded into T75 flasks and cultured in an MSC growth medium for 1 day. For the non-preconditioning MSC groups, the cells were cultured for 3 days with a fresh MSC growth medium. For the preconditioning MSC groups, cells were cultured in the MSC preconditioning medium for 3 days. The cells from all eight groups were washed three times with Dulbecco’s phosphate-buffered saline. The cells were trypsinized and seeded for each experiment, including cell proliferation, IL4 and PDGF-BB expression level, and osteogenic differentiation performed by alkaline phosphatase (ALP) staining and Alizarin red-staining. Abbreviations: MSCs, mesenchymal stromal cells; pMSCs, preconditioning MSCs; TNFα, tumor necrosis factor-alpha; LPS, lipopolysaccharide.

    Techniques Used: In Vitro, Cell Culture, Saline, Expressing, Staining

    Fold change in the amount of dsDNA compared to day 1.
    Figure Legend Snippet: Fold change in the amount of dsDNA compared to day 1.

    Techniques Used:

    ( A ) IL4 and PDGF-BB expression levels on day 3 in the IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups. IL4 expression levels on day 3 in the IL4-MSC, IL4-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 77.7 ± 4.4 ng/mL, 34.6 ± 5.7 ng/mL, 16.8 ± 9.4 ng/mL, and 41.8 ± 5.0 ng/mL, respectively. PDGF-BB expression levels on day 3 in the PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 121.7 ± 20.1 pg/mL, 50.3 ± 20.4 pg/mL, 8.7 ± 2.0 pg/mL, 71.5 ± 7.8 pg/mL, respectively. ( B ) IL4 and PDGF-BB expression level-dsDNA ratios on day 3 in the IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups. IL4 expression level-dsDNA ratios on day 3 in the IL4-MSC, IL4-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 7.3 ± 0.6, 1.8 ± 0.2, 1.8 ± 1.0, and 2.3 ± 0.1, respectively. PDGF-BB expression level-dsDNA ratios on day 3 in the PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 5.6 ± 0.9, 3.7 ± 1.4, 1.0 ± 0.3, and 3.9 ± 0.1, respectively.
    Figure Legend Snippet: ( A ) IL4 and PDGF-BB expression levels on day 3 in the IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups. IL4 expression levels on day 3 in the IL4-MSC, IL4-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 77.7 ± 4.4 ng/mL, 34.6 ± 5.7 ng/mL, 16.8 ± 9.4 ng/mL, and 41.8 ± 5.0 ng/mL, respectively. PDGF-BB expression levels on day 3 in the PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 121.7 ± 20.1 pg/mL, 50.3 ± 20.4 pg/mL, 8.7 ± 2.0 pg/mL, 71.5 ± 7.8 pg/mL, respectively. ( B ) IL4 and PDGF-BB expression level-dsDNA ratios on day 3 in the IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups. IL4 expression level-dsDNA ratios on day 3 in the IL4-MSC, IL4-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 7.3 ± 0.6, 1.8 ± 0.2, 1.8 ± 1.0, and 2.3 ± 0.1, respectively. PDGF-BB expression level-dsDNA ratios on day 3 in the PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 5.6 ± 0.9, 3.7 ± 1.4, 1.0 ± 0.3, and 3.9 ± 0.1, respectively.

    Techniques Used: Expressing

    Alkaline phosphatase-staining in all eight groups 2 weeks after seeding. The percentages of stained area in the GFP-MSC, GFP-pMSC, IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 19.8 ± 5.3%, 28.3 ± 1.4%, 4.3 ± 0.7%, 6.0 ± 2.4%, 44.2 ± 11.7%, 42.5 ± 8.1%, 21.4 ± 1.5%, and 25.7 ± 1.0%, respectively. Abbreviations: ALP, alkaline phosphatase.
    Figure Legend Snippet: Alkaline phosphatase-staining in all eight groups 2 weeks after seeding. The percentages of stained area in the GFP-MSC, GFP-pMSC, IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 19.8 ± 5.3%, 28.3 ± 1.4%, 4.3 ± 0.7%, 6.0 ± 2.4%, 44.2 ± 11.7%, 42.5 ± 8.1%, 21.4 ± 1.5%, and 25.7 ± 1.0%, respectively. Abbreviations: ALP, alkaline phosphatase.

    Techniques Used: Staining

    Alizarin red-staining in all eight groups 4 weeks after seeding. The percentages of stained area in the GFP-MSC, GFP-pMSC, IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 33.1 ± 4.7%, 45.1 ± 4.9%, 0.64 ± 0.08%, 0.58 ± 0.03%, 51.7 ± 14.5%, 40.7 ± 6.7%, 17.3 ± 2.5%, and 41.2 ± 1.7%, respectively.
    Figure Legend Snippet: Alizarin red-staining in all eight groups 4 weeks after seeding. The percentages of stained area in the GFP-MSC, GFP-pMSC, IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 33.1 ± 4.7%, 45.1 ± 4.9%, 0.64 ± 0.08%, 0.58 ± 0.03%, 51.7 ± 14.5%, 40.7 ± 6.7%, 17.3 ± 2.5%, and 41.2 ± 1.7%, respectively.

    Techniques Used: Staining

    The summary of the results.
    Figure Legend Snippet: The summary of the results.

    Techniques Used: Expressing



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    R&D Systems rabbit il4 elisa kit
    Representative fluorescence microscopy images of mesenchymal stromal cells (MSCs) infected by IL-4 and/or PDGF-BB lentiviral vectors. GFP-MSCs, MSCs infected with control GFP-positive lentivirus vector; <t>IL4-MSCs,</t> MSCs infected with rIL-4 secreting GFP-positive lentivirus vector; PDGF-BB-MSCs, MSCs infected with hPDGF-BB secreting GFP-positive lentivirus vector; IL4-PDGF-BB-MSCs, previously established IL4-MSCs infected with hPDGF-BB secreting RFP-positive lentivirus vector.
    Rabbit Il4 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit il4 elisa kit/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    rabbit il4 elisa kit - by Bioz Stars, 2026-06
    93/100 stars
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    Image Search Results


    Representative fluorescence microscopy images of mesenchymal stromal cells (MSCs) infected by IL-4 and/or PDGF-BB lentiviral vectors. GFP-MSCs, MSCs infected with control GFP-positive lentivirus vector; IL4-MSCs, MSCs infected with rIL-4 secreting GFP-positive lentivirus vector; PDGF-BB-MSCs, MSCs infected with hPDGF-BB secreting GFP-positive lentivirus vector; IL4-PDGF-BB-MSCs, previously established IL4-MSCs infected with hPDGF-BB secreting RFP-positive lentivirus vector.

    Journal: Bioengineering

    Article Title: Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

    doi: 10.3390/bioengineering8110165

    Figure Lengend Snippet: Representative fluorescence microscopy images of mesenchymal stromal cells (MSCs) infected by IL-4 and/or PDGF-BB lentiviral vectors. GFP-MSCs, MSCs infected with control GFP-positive lentivirus vector; IL4-MSCs, MSCs infected with rIL-4 secreting GFP-positive lentivirus vector; PDGF-BB-MSCs, MSCs infected with hPDGF-BB secreting GFP-positive lentivirus vector; IL4-PDGF-BB-MSCs, previously established IL4-MSCs infected with hPDGF-BB secreting RFP-positive lentivirus vector.

    Article Snippet: The expression levels of IL4 and PDGF-BB on day 3 were measured using the rabbit IL4 ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the human PDGF-BB ELISA kit (R&D Systems, Minneapolis), respectively, according to the manufacturer’s protocols.

    Techniques: Fluorescence, Microscopy, Infection, Control, Plasmid Preparation

    The experimental outline of in vitro experiments. GFP-MSCs, IL4-MSCs, PDGF-BB-MSCs, or IL4-PDGF-BB-MSCs were seeded into T75 flasks and cultured in an MSC growth medium for 1 day. For the non-preconditioning MSC groups, the cells were cultured for 3 days with a fresh MSC growth medium. For the preconditioning MSC groups, cells were cultured in the MSC preconditioning medium for 3 days. The cells from all eight groups were washed three times with Dulbecco’s phosphate-buffered saline. The cells were trypsinized and seeded for each experiment, including cell proliferation, IL4 and PDGF-BB expression level, and osteogenic differentiation performed by alkaline phosphatase (ALP) staining and Alizarin red-staining. Abbreviations: MSCs, mesenchymal stromal cells; pMSCs, preconditioning MSCs; TNFα, tumor necrosis factor-alpha; LPS, lipopolysaccharide.

    Journal: Bioengineering

    Article Title: Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

    doi: 10.3390/bioengineering8110165

    Figure Lengend Snippet: The experimental outline of in vitro experiments. GFP-MSCs, IL4-MSCs, PDGF-BB-MSCs, or IL4-PDGF-BB-MSCs were seeded into T75 flasks and cultured in an MSC growth medium for 1 day. For the non-preconditioning MSC groups, the cells were cultured for 3 days with a fresh MSC growth medium. For the preconditioning MSC groups, cells were cultured in the MSC preconditioning medium for 3 days. The cells from all eight groups were washed three times with Dulbecco’s phosphate-buffered saline. The cells were trypsinized and seeded for each experiment, including cell proliferation, IL4 and PDGF-BB expression level, and osteogenic differentiation performed by alkaline phosphatase (ALP) staining and Alizarin red-staining. Abbreviations: MSCs, mesenchymal stromal cells; pMSCs, preconditioning MSCs; TNFα, tumor necrosis factor-alpha; LPS, lipopolysaccharide.

    Article Snippet: The expression levels of IL4 and PDGF-BB on day 3 were measured using the rabbit IL4 ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the human PDGF-BB ELISA kit (R&D Systems, Minneapolis), respectively, according to the manufacturer’s protocols.

    Techniques: In Vitro, Cell Culture, Saline, Expressing, Staining

    Fold change in the amount of dsDNA compared to day 1.

    Journal: Bioengineering

    Article Title: Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

    doi: 10.3390/bioengineering8110165

    Figure Lengend Snippet: Fold change in the amount of dsDNA compared to day 1.

    Article Snippet: The expression levels of IL4 and PDGF-BB on day 3 were measured using the rabbit IL4 ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the human PDGF-BB ELISA kit (R&D Systems, Minneapolis), respectively, according to the manufacturer’s protocols.

    Techniques:

    ( A ) IL4 and PDGF-BB expression levels on day 3 in the IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups. IL4 expression levels on day 3 in the IL4-MSC, IL4-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 77.7 ± 4.4 ng/mL, 34.6 ± 5.7 ng/mL, 16.8 ± 9.4 ng/mL, and 41.8 ± 5.0 ng/mL, respectively. PDGF-BB expression levels on day 3 in the PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 121.7 ± 20.1 pg/mL, 50.3 ± 20.4 pg/mL, 8.7 ± 2.0 pg/mL, 71.5 ± 7.8 pg/mL, respectively. ( B ) IL4 and PDGF-BB expression level-dsDNA ratios on day 3 in the IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups. IL4 expression level-dsDNA ratios on day 3 in the IL4-MSC, IL4-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 7.3 ± 0.6, 1.8 ± 0.2, 1.8 ± 1.0, and 2.3 ± 0.1, respectively. PDGF-BB expression level-dsDNA ratios on day 3 in the PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 5.6 ± 0.9, 3.7 ± 1.4, 1.0 ± 0.3, and 3.9 ± 0.1, respectively.

    Journal: Bioengineering

    Article Title: Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

    doi: 10.3390/bioengineering8110165

    Figure Lengend Snippet: ( A ) IL4 and PDGF-BB expression levels on day 3 in the IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups. IL4 expression levels on day 3 in the IL4-MSC, IL4-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 77.7 ± 4.4 ng/mL, 34.6 ± 5.7 ng/mL, 16.8 ± 9.4 ng/mL, and 41.8 ± 5.0 ng/mL, respectively. PDGF-BB expression levels on day 3 in the PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 121.7 ± 20.1 pg/mL, 50.3 ± 20.4 pg/mL, 8.7 ± 2.0 pg/mL, 71.5 ± 7.8 pg/mL, respectively. ( B ) IL4 and PDGF-BB expression level-dsDNA ratios on day 3 in the IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups. IL4 expression level-dsDNA ratios on day 3 in the IL4-MSC, IL4-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 7.3 ± 0.6, 1.8 ± 0.2, 1.8 ± 1.0, and 2.3 ± 0.1, respectively. PDGF-BB expression level-dsDNA ratios on day 3 in the PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 5.6 ± 0.9, 3.7 ± 1.4, 1.0 ± 0.3, and 3.9 ± 0.1, respectively.

    Article Snippet: The expression levels of IL4 and PDGF-BB on day 3 were measured using the rabbit IL4 ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the human PDGF-BB ELISA kit (R&D Systems, Minneapolis), respectively, according to the manufacturer’s protocols.

    Techniques: Expressing

    Alkaline phosphatase-staining in all eight groups 2 weeks after seeding. The percentages of stained area in the GFP-MSC, GFP-pMSC, IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 19.8 ± 5.3%, 28.3 ± 1.4%, 4.3 ± 0.7%, 6.0 ± 2.4%, 44.2 ± 11.7%, 42.5 ± 8.1%, 21.4 ± 1.5%, and 25.7 ± 1.0%, respectively. Abbreviations: ALP, alkaline phosphatase.

    Journal: Bioengineering

    Article Title: Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

    doi: 10.3390/bioengineering8110165

    Figure Lengend Snippet: Alkaline phosphatase-staining in all eight groups 2 weeks after seeding. The percentages of stained area in the GFP-MSC, GFP-pMSC, IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 19.8 ± 5.3%, 28.3 ± 1.4%, 4.3 ± 0.7%, 6.0 ± 2.4%, 44.2 ± 11.7%, 42.5 ± 8.1%, 21.4 ± 1.5%, and 25.7 ± 1.0%, respectively. Abbreviations: ALP, alkaline phosphatase.

    Article Snippet: The expression levels of IL4 and PDGF-BB on day 3 were measured using the rabbit IL4 ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the human PDGF-BB ELISA kit (R&D Systems, Minneapolis), respectively, according to the manufacturer’s protocols.

    Techniques: Staining

    Alizarin red-staining in all eight groups 4 weeks after seeding. The percentages of stained area in the GFP-MSC, GFP-pMSC, IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 33.1 ± 4.7%, 45.1 ± 4.9%, 0.64 ± 0.08%, 0.58 ± 0.03%, 51.7 ± 14.5%, 40.7 ± 6.7%, 17.3 ± 2.5%, and 41.2 ± 1.7%, respectively.

    Journal: Bioengineering

    Article Title: Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

    doi: 10.3390/bioengineering8110165

    Figure Lengend Snippet: Alizarin red-staining in all eight groups 4 weeks after seeding. The percentages of stained area in the GFP-MSC, GFP-pMSC, IL4-MSC, IL4-pMSC, PDGF-BB-MSC, PDGF-BB-pMSC, IL4-PDGF-BB-MSC, and IL4-PDGF-BB-pMSC groups were 33.1 ± 4.7%, 45.1 ± 4.9%, 0.64 ± 0.08%, 0.58 ± 0.03%, 51.7 ± 14.5%, 40.7 ± 6.7%, 17.3 ± 2.5%, and 41.2 ± 1.7%, respectively.

    Article Snippet: The expression levels of IL4 and PDGF-BB on day 3 were measured using the rabbit IL4 ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the human PDGF-BB ELISA kit (R&D Systems, Minneapolis), respectively, according to the manufacturer’s protocols.

    Techniques: Staining

    The summary of the results.

    Journal: Bioengineering

    Article Title: Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

    doi: 10.3390/bioengineering8110165

    Figure Lengend Snippet: The summary of the results.

    Article Snippet: The expression levels of IL4 and PDGF-BB on day 3 were measured using the rabbit IL4 ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the human PDGF-BB ELISA kit (R&D Systems, Minneapolis), respectively, according to the manufacturer’s protocols.

    Techniques: Expressing